Composite

Part:BBa_K3747138:Design

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


napEDABC (Periplasmic nitrate reductase) Cupriavidus necator H16


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1557
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
    Illegal NotI site found at 1044
    Illegal NotI site found at 2884
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1557
    Illegal BglII site found at 539
    Illegal BglII site found at 571
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1557
    Illegal PstI site found at 1497
    Illegal PstI site found at 1962
    Illegal PstI site found at 3015
    Illegal PstI site found at 3792
    Illegal NgoMIV site found at 153
    Illegal NgoMIV site found at 288
    Illegal NgoMIV site found at 1125
    Illegal NgoMIV site found at 1261
    Illegal NgoMIV site found at 1856
    Illegal NgoMIV site found at 2613
    Illegal NgoMIV site found at 2684
    Illegal AgeI site found at 1312
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Cupriavidus necator H16

References